Twenty three of the 30 loci were amplified and 17 loci yielded high success rate (> 91%). We obtained blood samples of 57 Indian ducks (Anas platyrhynchos) belonging to three indigenous duck populations of geographically distinct locations of the country and genotyped them using chicken microsatellite markers.
Therefore, more effort should be committed to developing quail-specific markers rather than attempting to adapt chicken markers for work in quail. Although the very few chicken markers that do work well in quail could be used as anchor points for a comparative mapping, most chicken markers are not useful for studies in quail. For these six loci, allele frequencies were estimated in 50 unrelated quails. The sequence information of the remaining nine loci revealed six of them to contain microsatellites that were nearly identical with those of the orthologous regions in chicken. Eleven of the polymorphic loci were excluded as uninformative because of the lack of amplification in some individuals or high frequency of nonspecific amplification. Twenty-six per cent (31/120) of chicken primers amplified individual loci in Japanese quail and 65% (20/31) of the amplified loci were found to be polymorphic. We tested chicken microsatellite markers to see if they would be suitable as genetic linkage markers in Japanese quail. The exchange of marker information between chicken and quail is an important step towards the construction of a high-resolution comparative genetic map in Phasianidae, which includes several poultry species of agricultural importance. Therefore, a significant portion of chicken microsatellite markers can be useful for genomic mapping and linkage analysis in the turkey, reducing the costs involved in producing turkey-specific microsatellite markers.ĭomestic fowl or chicken (Gallus gallus) and Japanese quail (Coturnix japonica) belong to the family Phasianidae. Among 18 primerpairs tested for polymorphism, using three commercial turkey lines, five were found to exhibit length polymorphism, three of which did not contain a detectable TG repeat. Hybridization using end-labelled (TG)8 as a probe showed that, out of 41 primer-pairs tested, only 14 generated an amplification product that also contained a detectable (TG)n microsatellite repeat when turkey DNA was the template. Results indicated that the majority (92%) of these primer-pairs generated amplification products in turkey genomic DNA.
These primer-pairs were used in the polymerase chain reaction to amplify turkey (genus Meleagris) genomic DNA loci. Forty-eight primer-pairs complementary to unique DNA sequences flanking chicken (genus Gallus) genomic (TG)n microsatellite repeats were previously designed.
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